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Research finds that using SMRT sequencing technology to identify liquid enzyme strains is more reliable
Author: admin Date: 2018-07-20 Views: 82

The study found that the use of SMRT sequencing technology for liquid enzyme strain identification has more reliable results

基因为标记基因,基于SMRT测序技术对16sRNA基因进行分型,从而实现对液体酵素中微生物在种水平的鉴定和丰度分析;具体步骤包括:液体酵素DNA的提取, PCR扩增,构建2K文库,生物信息学分析,菌种鉴定和丰度分析,实现了液体酵素中微生物在种水平的鉴定和丰度分析,与现在技术相比,该方法不仅能应对相对复杂的样本,并且鉴定的结果可靠性更高。 Using SMRT sequencing technology is a method for identifying liquid enzyme strains. This method uses 16sRNA gene as a marker gene and types 16sRNA gene based on SMRT sequencing technology to achieve the identification and abundance of microorganisms in liquid enzyme at the species level. Analysis; the specific steps include: extraction of liquid enzyme DNA , PCR amplification, construction of a 2K library, bioinformatics analysis, strain identification and abundance analysis, and the identification and abundance analysis of microorganisms in liquid enzyme at the species level, Compared with current technology, this method can not only deal with relatively complex samples, but also has higher reliability in identification results.

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